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New Products

J&K Scientific is committed to research and innovation. We are proud of our independent new product achievements as well as our development partnership with our customers and distributors. For many years J&K has invited companies to collaborate and share in market opportunities by contributing and co-developing products. Our product range grows every year with thousands of new compounds and derivatives added to our existing list of over 500,000 ultrafine materials, pharmaceutical chemicals, biochemicals and specialty chemical products for science and industry.
  • Latest Products
  • March 15 2018
  • December 15 2017
  • November 30 2017

Novel ‘Independence’ Mitophagy Fluorescent Probe HQO

Mitochondria are the energy factories of cells; their function involves a number of important physiological processes such as cell metabolism, signaling, differentiation, growth, and apoptosis. Mitophagy is the process by which cells remove damaged or senescent mitochondria and circulate their constituent elements. Therefore, the study of mitophagy is of great significance. Currently, the study of mitophagy is done mainly by antibody or fluorescent protein labeling methods, or mitochondrial fluorescence probe and lysosome fluorescence probe combined detection. However, the former is a complex operation with limited application in living cells, while the latter has limited specificity.

The pH-sensitive fluorescent probe HQO does not need to be used in combination with other probes, and can enter the living cells for selective enrichment in mitochondria. When the mitochondria undergo autophagy to form autophagy lysosomes, the microenvironmental pH decreases, leading to HQO protonation; the fluorescence excitation and emission wavelengths of protonated HQO all undergo red shift (Stokes shift greater than 100 nm). Due to the different pH values in the microenvironment, HQO shows different colors of fluorescence in mitochondria and autophagy lysosomes. The normal mitochondria and damaged mitochondria coated with autophagy lysosomes can thereby be clearly distinguished. HQO molecules can accurately locate mitochondria and accurately trace mitophagy. This probe molecule provides a new research method for studying mitochondrial metabolism and provides an effective tool for screening mitophagy inhibitors and accelerators.

HQO has two excitations and corresponding emission wavelengths, one tracer mitochondria, one tracer autophagy lysosome, and offers elucidation of autophagic flow processes through two wavelength changes. Compared with conventional autophagy detection methods, it has the following advantages:
      1. Simpler sample preparation;
      2. Less steps, supplies and tools;
      3. Specificity clearly indicates the process whereby mitochondria are phagocytosed to form autophagy lysosomes, and can accurately indicate that local mitochondria phagocytosed;




Fig.1 HQO and HQO tracing live mitophagy process schematic



Instructions for use of HQO probe:
      1) Cell culture: Cells are planted in confocal Petri dishes. Cell density and culture time vary according to different experiment parameters.
      2) Induction of autophagy and cell staining: Induction of autophagy in appropriate conditions (such as serum-free medium, PBS or autophagic activator treatment, etc.). HQO (10 μM) in serum-free medium is added to the culture dish and incubated at 37 ° C for 10 min, washed one to three times with PBS.
      3) Confocal imaging: 559 nm excitation, 570-610 nm emission wavelength for tracing the fluorescence of normal mitochondria; 635 nm excitation, 650-730 nm emission wavelength for tracing the fluorescence of autophagy lysosomes.

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structure Product no. description cas pricing
2668725 2,6-bi(2-(3,3-dimethyl-1-propyl-indolin-2-ylidene)ethylidene)-cyclohexanone, 98% 1442645-34-2 Pricing


Other fluorescent probe products:

structure Product no. description cas pricing
2668724 2,9-bis[4-(4-methylpiperazin-1-yl)styryl]-1,10-phenanthroline, 98% 1792998-33-4 Pricing
2668727 CSTS, 98% Pricing
433521 D-Luciferin potassium salt, 98% 115144-35-9 Pricing
2668726 3,9-dihydro-1,3-dioxopyrido[3,4,5-gh][1,2,4]triazolo[1,5-a]perimidine-2(1h)-hexanoic acid, 98% 1359819-97-8 Pricing
102273 2,3-Diaminonaphthalene, 97% 771-97-1 Pricing
2668723 9-methyl-3,6-bis[5-(4-methylpiperazin-1-yl)-1h-benzo[d]imidazol-2-yl]-9h-carbazole, 98% 1514901-89-3 Pricing
227585 o-Phthalaldehyde, 99% 643-79-8 Pricing

High ratio-responsive pH fluorescent ANNA probe

Intracellular and extracellular pH changes play an important role in physiological and pathological processes. Changes in intracellular pH can easily induce cell division, rapid growth and dysfunction. Reduced extracellular pH is an important signal for cancers and other pathologies. The detection of pH in different regions inside and outside the cell is helpful to study cell life processes and pathological mechanisms.

ANNA is an ideal ratio pH fluorescent probe:
High Sensitivity: ANNA absorption and emission spectra in the physiological pH range (pH 6-8), offers high quantum yield fluorescence in both acidic and alkaline environments;
Good Reactivity: The carboxyl group on ANNA can be coupled to a variety of other biological molecules, drug molecules or micro-nano materials, and its pH response function is not affected;
The reason is that the probe pH response comes from the 4H-1,2,4-triazole (1,5-a) diazacyclo-1,8-naphthalimide structure, and the fluorescence signal is not affected by the change in the acyl imine N-substituents;
Favorable stability: The pH response has good reversibility and can be used repeatedly for a long time; It is very stable when exposed in light and air;
High anti-interference ability and wide range of applications: The fluorophore of this probe is not disturbed by ionic strength; therefore, it can be used in complex biological systems of cells and animals.

Instructions for use of ANNA probe:
1) Cells are cultured in 20 mm diameter glass basal cell culture dishes (MatTek Co.) for 24 h prior to imaging;
2) The pH probe ANNA (4 μM) is incubated with the cells for 1-2 h in cell culture medium;
3) Cells are washed three times with PBS buffer (pH = 7.4) to remove free probes;
4) Cell imaging is observed with a 100x confocal microscope. With excitation wavelengths of 405 nm and 488 nm, fluorescence signals collected intensities at 425-475 nm for channel 1 and 500-600 nm for channel 2. (If equipped with 455nm laser: 455nm can be directly used to stimulate the collection of 1 channel (470nm-500nm) and 2 channel (505nm-540nm) fluorescence intensity.)
5) pH standard curve: The cells aretreated with 1 mL of high K + HEPES buffer of different pH with 5 ug / mL nigericin (a carrier carrying potassium ions and hydrogen ions, enabling fast and consistent intracellular and extracellular pH) for 10 min. High K + HEPES buffer contains 125 mM KCl, 20 mM NaCl, 0.5 mM CaCl2, 0.5 mM MgCl2, 5 mM glucose and 20 mM HEPES. The cells are imaged, the curve is drawn according to the relationship between the channels I2 / I1 and the pH. The ratio of the sample to be tested is brought into the standard curve to obtain the actual cell pH.

+More


structure Product no. description cas pricing
2668726 3,9-dihydro-1,3-dioxopyrido[3,4,5-gh][1,2,4]triazolo[1,5-a]perimidine-2(1h)-hexanoic acid, 98% 1359819-97-8 Pricing


Other fluorescent probes:

structure Product no. description cas pricing
2668725 2,6-bi(2-(3,3-dimethyl-1-propyl-indolin-2-ylidene)ethylidene)-cyclohexanone, 98% 1442645-34-2 Pricing
2668724 2,9-bis[4-(4-methylpiperazin-1-yl)styryl]-1,10-phenanthroline, 98% 1792998-33-4 Pricing
2668727 CSTS, 98% Pricing
433521 D-Luciferin potassium salt, 98% 115144-35-9 Pricing
102273 2,3-Diaminonaphthalene, 97% 771-97-1 Pricing
2668723 9-methyl-3,6-bis[5-(4-methylpiperazin-1-yl)-1h-benzo[d]imidazol-2-yl]-9h-carbazole, 98% 1514901-89-3 Pricing
227585 o-Phthalaldehyde, 99% 643-79-8 Pricing

3BDO—A new autophagy inhibitor

MTOR, a central regulator of autophagy, is involved in cancer and cardiovascular and neurological diseases. Modulating the MTOR signaling balance could be of great significance for numerous diseases. In previous studies, Professor BaoXiang Zhao of Shandong University found a chemical small molecule , 3-Benzyl-5-((2-nitrophenoxy) methyl)–dihydrofuran-2(3H)-one (3BDO), that inhibited autophagy in human umbilical vein endothelial cells (HUVEC s) and neuronal cells. JK Scientific offers 3BDO as sole agent.


3BDO was more efficient than 3-MA as autophagy inhibitor

3BDO could dock on FKBP1A in the same sites (TYR82A and ILE56A) as Rapamycin


Figure 1: Conceptual schematic of 3BDO and FLJ11812 mechanism of action


+More
structure Product no. description cas pricing
1914077 3BDO, 97%, Activator for mTOR, Autophagy inhibitor 890405-51-3 Pricing
369172 6-Amino-3-methylpurine, 90% 5142-23-4 Pricing
948477 Rapamycin, 98% 53123-88-9 Pricing

Reagents for Photoresists Synthesis

Photoresists are widely used in integrated circuits, semiconductor devices, touch screens and photovoltaic materials. As a professional supplier, J&K provides a variety of reagents for the synthesis of photoresists. Our products offer reliable quality and high performance and are available in different package sizes from grams to kilograms to meet your requirements from basic research to large-scale manufacturing.

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Monomers for Photoresists:

structure Product no. description cas pricing
476032 1,3-Adamantanediacetic acid, 97% 17768-28-4 Pricing
191866 Dichlorodimethylsilane, 99.3% 75-78-5 Pricing
313268 Chlorendic anhydride, 96% 115-27-5 Pricing
396012 Isobornyl acrylate, 93%, stabilized with 200 - 400 ppm MEHQ 5888-33-5 Pricing
609785 cis-5-Norbornene-exo-2,3-dicarboxylic anhydride, 96% 2746-19-2 Pricing
339028 n-Octyltriethoxysilane, 97% 2943-75-1 Pricing
953647 Trichloro(octyl)silane, 97% 5283-66-9 Pricing

Additives for Photoresists:

structure Product no. description cas pricing
908824 Butyl acetate, 99% 123-86-4 Pricing
277506 9-Fluorenylmethyl carbamate, 98% 84418-43-9 Pricing
126667 2-Hydroxyethyl methacrylate, 99%, stabilized with 100 ppm MEHQ 868-77-9 Pricing
193900 Pyrogallol, 99% 87-66-1 Pricing
496960 Trimethylolpropane trimethacrylate, 90% 3290-92-4 Pricing

Photoacid Generators:

structure Product no. description cas pricing
512472 Bis(4-tert-butylphenyl)iodonium triflate, 98% 84563-54-2 Pricing
517519 Diphenyliodonium nitrate, 97% 722-56-5 Pricing
305170 2-?(4-?Methoxystyryl)?-?4,6-?bis(trichloromethyl)?-?1,3,5-?triazine, 98% 42573-57-9 Pricing

Photoinitiator for Photoresists:

structure Product no. description cas pricing
167389 Acetophenone, 98% 98-86-2 Pricing
248099 Benzophenone, 99% 119-61-9 Pricing
606200 4,4'-Bis(diethylamino)benzophenone, 99% 90-93-7 Pricing
543244 2,2-Dimethoxy-2-phenylacetophenone, 98% 24650-42-8 Pricing

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